Estimating the number of channels in patch recordings

Nanoscale-Targeted Patch-Clamp Recordings of Functional Presynaptic Ion Channels

Direct electrical access to presynaptic ion channels has hitherto been limited to large specialized terminals such as the calyx of Held or hippocampal mossy fiber bouton. The electrophysiology and ion-channel complement of far more abundant small synaptic terminals (≤ 1 μm) remain poorly understood. Here we report a method based on superresolution scanning ion conductance imaging of small synap...

Estimating the Number of Wideband Radio Sources

In this paper, a new approach for estimating the number of wideband sources is proposed which is based on RSS or ISM algorithms. Numerical results show that the MDL-based and EIT-based proposed algorithm havea much better detection performance than that in EGM and AIC cases for small differences between the incident angles of sources. In addition, for similar conditions, RSS algorithm offers hi...

Patch Recordings from the Electrocytes

Sodium currents were recorded in cell-attached and inside-out patches from the innervated membrane of Electrophorus electrocytes. Electrocytes from Sachs and main electric organs were prepared as described by Pasquale et al. (1986.J. Membr. Biol. 93:195.). Maximal currents in the Sachs organ, measured with 1-2-p.m diameter patch pipettes and at room temperature, were in the range of 20 to 300 p...

Errors in the measurement of voltage-activated ion channels in cell-attached patch-clamp recordings

Patch-clamp recording techniques have revolutionized understanding of the function and sub-cellular location of ion channels in excitable cells. The cell-attached patch-clamp configuration represents the method of choice to describe the endogenous properties of voltage-activated ion channels in the axonal, somatic and dendritic membrane of neurons, without disturbance of the intracellular milie...

Critical stability number in rock lined channels